RNA Interference in Practice

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RNA Interference in Practice

In the short time since its elucidation, RNA interference (RNAi) has quickly emerged as the premier tool in which to study gene function. RNAi provides a simple and predictable yet extremely powerful and specific method in which to knock down gene expression in virtually any cell type or whole organism. The major advance in RNAi technology, particularly for mammalian systems, came with the disc...

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Enhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase

Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...

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an introduction to rna interference (rnai)

the discovery of rna interference (rnai) in recent years has revolutionized biological and medical research. rnai is the sequence-specific gene silencing triggered by double-stranded rna (dsrna). until a couple of years ago only biologists working with caenorhabditis elegans and plants could use rnai technology to gain insight into gene function……

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enhancement of rna interference effect in p19 ec cells by an rna-dependent rna polymerase

background: rna interference (rnai) is a phenomenon uses double-stranded rna (dsrna) to specifically inhibit gene expression. the non-specific silencing caused by interferon response to dsrna in mammalian cells limits the potential of utilizing rnai to study gene function. duplexes of 21-nucleotide short interfering dsrna (sirna) inhibit gene expression by rnai. in some organisms, sirna can als...

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ژورنال

عنوان ژورنال: Comparative and Functional Genomics

سال: 2005

ISSN: 1531-6912,1532-6268

DOI: 10.1002/cfg.487